nCounter® Analysis System

One Powerful, Fully Automated System for Translational Research

The nCounter® Analysis System offers a simple, cost-effective way to profile hundreds of mRNAs, microRNAs, or DNA targets simultaneously with high sensitivity and precision. The digital detection of target molecules and high levels of multiplexing eliminate the compromise between data quality and data quantity, producing gold-standard sensitivity and reproducibility for studies of hundreds of targets. The system uses molecular “barcodes” and single molecule imaging to detect and count hundreds of unique tran스크립트s in a single reaction. Unlike other methods, the protocol does not include any amplification steps that might introduce bias to the results.

NEW High Sensitivity Protocol - (Prep Station software update)

Our most recent update is focused on optimizing the binding of the purified probe-target tripartite complex to the slide surface, which results in higher and more reproducible digital counts per sample. This optimization involves the priming of the Cartridge binding surface prior to sample introduction combined with extending the time provided for binding of the probe-target complex to the slide surface.
 - Increased fold-change sensitivity
Increased system sensitivity (more genes detected)
Reduced input material option
(50ng for gene expression and miRNA applications; 150ng for CNV)

Performance
* No amplification, no RT, no bias
* High sensitivity, precision, digital quantification
* GMP/ISO 13485 compliant

Easy-to-Use
* Fully-automated
* Intuitive user interface
* 15-minutes hands-on time

Efficient
* Multiplex up to 800 targets in a single reaction
* Analyze FFPE, lysates, total RNA
* Less bench-time, faster time to study completion

System Specifications
Specifications                               Prep Station                  Digital Analyzer
Operating Temperature                     18°C - 28°C                      18°C - 28°C
Humidity                             20 - 80% (non-condensing)    20 - 80% (non-condensing)
Pollution degree                                  2                                     2
Power source                          100-240VAC, 50-60 Hz        100-240VAC, 50-60 Hz
Power draw                                     610VA                              610VA
Dimensions                               67 x 89 x 63 cm                  66 x 66 x 48 cm
Weight                                            120 kg                             120 kg
Warranty                                          1 year                             1 year

System Performance
Level of multiplexing : Up to 800 nucleic acid targets per assay
Recommended amount of starting material : 100ng of total RNA, or lysate from ~10,000 cells
Sample types supported : Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene lysed whole blood
Reaction volume : 30 μL
Limit of detection : 0.5fM spike-in control
Fold change sensitivity : > 1.5 fold (> 5 copies per cell);
                                   > 2 fold change (> 1 copy per cell)
Spike correlation : R² ≥ 0.95
Linear dynamic range : 7 x 10⁵ total counts
Prep Station throughput : 12 samples < 2.5 hours
Digital Analyzer throughput : 12 samples per 2.7 hours
Controls : 6 positive and 8 negative in each reaction